Ulcerative colitis is chronically inflammatory bowel disease of unknown origin. This study was undertaken to determine the healing of ulcerative colitis induced by acetic acid due to antioxidant role of kalasakadi kashayam. Kalasakadi kashayam is an ayurvedic proprietary formulation manufactured by Arya Vaidya Sala, kottakkal with proven clinical efficacy. It contains kalasaka (Murraya koenigii Spreng.), Haritaki (Terminalia chebula Retz.) and Sunthi (Zingiber officinale Roxb.). Ulcerative colitis was induced by acetic acid in a dose 2 ml/kg (4% V/V Intrarectal). Ulcerative colitis response was assessed by ulcer index, tissue biochemical parameters, and histopathological examination of colon. The cytoprotective activity of kalasakadi kasayam was assessed at two dose level in comparison to sulfasalazine (360 mg/kg p.o.) as a reference standard drug. Therapeutically Equivalent Dose (TED) of kalasakadi kashayam was 5.4 ml/kg p.o. and double to TED was 10.8 ml/kg p.o. TED and TED × 2 doses restore the decrease level of superoxide dismutase, total glutathione, glutathione peroxidase and increase level of lipid peroxidation, nitric oxide, lactate dehydrogenase, myeloperoxidase activity. In addition kalasakadi kashayam at both dose levels showed significant decrease in ulcer index and histopathological changes of colon. Based on data generated and histopathological profile, it is concluded that kalasakadi kashayam TED level provide more significant protection than kalasakadi kashayam TED × 2 level against acetic acid induced ulcerative colitis.
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